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2.7. Measurement of Cellular Lipid ROS by Flow Cytometry
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Lipid ROS generation was measured by flow cytometry. The collected samples were stained with 10 µM of C11-BODIPY in the dark at 37 °C for 30 min. The suspended cells were transferred to a 5 mL FACS tube and determined on a flow cytometer (LSRFortessa, Becton Dickinson, Lexington, KY, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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