Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.1. 11α-OH-KA

IB Irmanida Batubara
ask Ask a question
Favorite

A. lavenia was collected from Bogor, West Java, Indonesia. 11αOH-KA, isolated from A. lavenia leaves with a purity of >95%, judging from the NMR spectra in Gifu University [4], was dissolved in dimethyl sulfoxide (DMSO). Briefly, a dried powder (100 g) obtained from A. lavenia leaves was soaked in distilled water 1:30 (w/v) at 55 °C for 12 h. The water-soluble fraction (A. lavenia water fraction) was recovered after filtration with coffee filtrates, and 11αOH-KA was purified by chloroform extraction (A. lavenia chloroform fraction) followed by silica gel chromatography. 11αOH-KA was recovered as crystals.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A