2.3. Growth inhibition assay

The inhibitory effect of the plant extracts on the radial mycelial growth of the selected fungi was assessed by the agar dilution method, as described before (Balouiri et al., 2016). Briefly, the sterile aqueous extracts were mixed, equally, with molted cooled (40–45 °C) Czapak Dox agar medium (Sigma-Aldrich, Saint Louis, Mo, USA) and different working solution (0, 10, 25 or 50 mg/ml) were prepared. The mixtures were poured into sterile Petri dishes and left for solidification. Later, a disc of 6 mm was isolated from the edge of the active growing colonies of each fungus, placed in the center of the prepared agar plates, and then incubated for 7 days at 25 ± 2 °C incubators; each assay was performed in triplicates. The radical mycelial growth was evaluated by calculating the mean of two perpendicular colony diameters for each replicate after the incubation time. The percentage of mycelial growth inhibition was calculated according to the following formula:

where DC and DT are the diameter of the control and treated colonies, respectively.