2.8. Experimental induction of diabetic neuropathy

Stimulation of diabetes was done through the single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg b.w. in 0.1 M citrate buffer (pH = 4.5) that was freshly prepared to rats after overnight fasting. After three weeks rats with high glucose level (more than 250 mg/dl) having glycosuria and hyperglaycemia, were considered as diabetic neuropathy rats and used for the experiment.