2.8. Cell treatments

Ly294002 (Sigma Aldrich) was used as a PI3K inhibitor at a concentration of 25 µM for 1 hour. MK‐2206 (2.5 µM, Selleck Chemicals, Houston, TX, USA) was used as a second Akt inhibitor. SC79 (10 µM, Sigma Aldrich) was used as an Akt activator for 24 hours. PDGFBB (PMG0041; Thermo Fisher Scientific) was used at 10 ng/mL concentration in DMEM without serum for the indicated times. Plates or chamber slides were coated with fibronectin at 1 ng/mL concentration overnight in PBS. They were then blocked in 0.5% of heat‐inactivated BSA at 37° for 30 minutes before cells were plated.