Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Primary culture of neonatal rat hippocampal neurons (NRNs)

MM Meng Mao
YX Yi Xu
XZ Xin-Yu Zhang
LY Lin Yang
XA Xiao-bin An
YQ Yang Qu
YC Ya-ni Chai
YW Yan-Ru Wang
TL Ting-ting Li
JA Jing Ai
request Request a Protocol
ask Ask a question
Favorite

Primary neuron cultures were prepared as previously described [31]. In brief, the hippocampus were removed from postnatal day 0 (P0) male SD rat pups and dissociated cells were maintained in DMEM containing 10% fetal bovine serum (FBS, HyClone, Logan, UT, USA) and subsequently inoculated into a poly-d-lysine precoated 6-well plate at a density of 1–2 × 106 cells/well. After 4 h of incubation, the culture medium was replaced with neurobasal medium (Gibco, USA) with 2% B27 supplement (Invitrogen, USA). Cultures were placed in a 37 °C humidified atmosphere with 5% CO2. Neurons were collected from days 5–7 of in vitro culture were used for the experiments.

Copyright and License information: The Author(s) ©2020
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A