Body weight was recorded daily. On days 0 and 14, stool samples were collected and evaluated for occult blood by using Hemoccult-II® SENSA® elite test strips (Beckman Coulter, Brea, CA, USA). Scoring was conducted in accordance with the manufacturer’s procedures. Necropsy was performed on day 21. The animals were anaesthetized by intraperitoneal administration of 5% isoflurane. The colon was excised, and its length was determined for each animal. The colon was weighed after removal of its contents and then partitioned for molecular analysis. DAI was calculated according to weight loss, colon weight/length ratio, and intestinal bleeding.
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