Assessment of colitis

PC Pedro A. Ruiz Castro
UK Ulrike Kogel
GS Giuseppe Lo Sasso
BP Blaine W. Phillips
AS Alain Sewer
BT Bjorn Titz
LG Llenalia Garcia
AK Athanasios Kondylis
EG Emmanuel Guedj
DP Dariusz Peric
DB David Bornand
RD Remi Dulize
CM Celine Merg
MC Maica Corciulo
NI Nikolai V. Ivanov
MP Manuel C. Peitsch
JH Julia Hoeng
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Body weight was recorded daily. On days 0 and 14, stool samples were collected and evaluated for occult blood by using Hemoccult-II® SENSA® elite test strips (Beckman Coulter, Brea, CA, USA). Scoring was conducted in accordance with the manufacturer’s procedures. Necropsy was performed on day 21. The animals were anaesthetized by intraperitoneal administration of 5% isoflurane. The colon was excised, and its length was determined for each animal. The colon was weighed after removal of its contents and then partitioned for molecular analysis. DAI was calculated according to weight loss, colon weight/length ratio, and intestinal bleeding.

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