Laser Doppler Imager (Moor Instruments) was used to serially monitor the blood perfusion recovery of the ischemic hind limbs. Briefly, mice were anesthetized and then imaged using Laser Doppler Imager. The blood flux was quantified using perfusion ratio — ratio of average laser Doppler perfusion imaging index of ischemic to nonischemic (contralateral, self-control) hind limbs — by MoorLDI V6 PC software (Moor Instruments).
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