p53 luciferase reporter assay

To determine the transcriptional activity of p53, MESOV cells were transfected with PG13-Luc plasmids. Approximately 5,000 transfected cells were seeded on to each well of 96-well plates and incubated overnight. These cells were treated with either control solvent, 5 μM SHetA2 or 60 μM PRIMA-1^MET for 24 hr. Luciferase activity of the reporter was measured with the One-Glo luciferase reporter assay kit (Promega, Madison, WI) and a Biotek Synergy H1 plate reader (BioteK, Winooski, VT). The MTT assay was performed in parallel cultures and used to normalize the p53 reporter activity to the number of viable cells.