Nerve Conduction Velocity Measurements

After anesthetizing the rats with sodium pentabarbitone (60 mg/kg b.w. i.p) from each group, sciatic nerve was exposed and bipolar needle electrodes (PowerLab, AD instrument, Model: ML825, Serial: 225-0907, Australia, frequency: 20 Hz, amplitude: 1.5 V and duration: 0.1 ms) were placed at two sides of sciatic nerve. Rectal temperature continued (37 ^oC) with a warm blanket tissue, drying was avoided by adding mammalian ringer solution. Evoked potential response (m/s) was calculated by dividing the distance (mm) and the difference in time (ms) between two stimulated points.⁴³