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Plasma free amino acids were assessed by ultra-performance liquid chromatography as previously described (29) with l-norvaline as an internal standard. The ultra-performance liquid chromatography was organized with a Thermo Scientific Dionex Ultimate 3000 pump, auto sampler, column oven, fluorescence detector (ThermoFisher Scientific), and a Kinetex C18 column headed by a Krudkatcher inline filter (Phenomenex). Chromeleon 7.1 software (ThermoFisher Scientific) was used to capture data.
Copyright and License information: ©2019 American Society for Nutrition 2019.
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