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Blood smears were made, air dried and stained with HAEMA quick stain (DIFF Quick, Labor + Technik, Eberhard Lehmann GmbH, Germany, No. LT001), following the manufacturer’s instructions. In the stained smears, the amount of segmented and band neutrophils (in 100 neutrophils) were counted using a light microscope (LEICA DM IL LED, HI Plan I 40×/0.50 PH2 objective) and the percentage in each sample was calculated. Example pictures were made with a Zeiss Axio Imager M2 microscope with a Plan-Apochromat 63×/1.4 Oil DIC ∞/0.17 objective.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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