Synthesized plasmids of 12 common viral pathogens, including SARS, MERS, influenza A H1N1/H3N2, influenza B, human parainfluenza viruses (HPIV-1/2/3), respiratory syncytial virus (RSV-A/B), Epstein-Barr virus, human cytomegalovirus, human mastadenovirus (HAdV-B/E), enterovirus (EB-U/71), human rhinovirus (HRV-2/14/16), and coxsackievirus (CA16), were used to test potential cross-reactivity in the developed RT-LAMP assay. The RT-LAMP products obtained using these plasmid templates were assayed by 3% agarose gel electrophoresis.
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