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Measurement of transepithelial electrical resistance (TEER)
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TEER was measured using a commercial electrical resistance system (Millicell; Millipore) in ARPE-19 and primary porcine RPE monolayers grown on Transwell filters as described above. TEER values were calculated by subtracting the value of a blank (transwell filter without cells). Measurements were repeated at least three times for each filter, and each experiment was repeated at least five times using 2 filters.
Copyright and License information: ©2020 Romero-Vázquez et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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