Four male WT BALB/c and seven male Siglecg−/− (Siglecgtm1Lnit, MGI:3718495) mice from two littermate sets between 16 and 18 wk old received half-dose FA-AKI (125 mg/kg FA). Extravascular kidney B cells were gated as single, live, in vitro CD45+, intravascular CD45−, CD19+ events. Extravascular kidney neutrophils were gated as single in vitro CD45+, intravascular CD45−, GR1+, CD11b+ events. Extravascular kidney monocytes were gated as single, live, in vitro CD45+, intravascular CD45−, Ly-6ClowMHCIIhigh events.
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