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Concentration of CPC and beta-lactam biosynthesis intermediates in the culture broth were determined in the CTAB/ acetonitrile/ orthophosphoric acid/ water mobile phase on a YMC-Pack ODS-A chromatographic column (“YMC CO.”, Japan) with a particle diameter of 5 μm at a flow rate of 1.0 ml/ min of the mobile phase, detection 254 nm.
Copyright and License information: ©2020 Zhgun et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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