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Peptide p4 was chemically synthesized by ChinaPeptide (Shanghai, China) at ≥95% purity. Biotin- or FITC-labeled p4 were synthesized by CASLO (Kongens Lyngby, Denmark) at ≥95% or ≥98% purity. Biotin was added directly at the N-terminus of p4. For FITC-labeled p4, C-terminal lysine was added to p4 and FITC was conjugated to the side chain of this C-terminal lysine. Both biotin-labeled and FITC-labeled p4 displayed similar antimicrobial activity to unmodified p4.
Copyright and License information: ©2020 Godlewska, Bilska, Majewski, Pyza, Zabel and Cichy.
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