Phylogenetic analysis

JN Jun Ni
HL Hanliang Lin
XX Xiaofeng Xu
QR Qiaoyun Ren
MA Malike Aizezi
JL Jin Luo
YL Yi Luo
ZM Zhan Ma
ZC Ze Chen
YT Yangchun Tan
JG Junhui Guo
WL Wenge Liu
ZQ Zhiqiang Qu
ZW Zegong Wu
JW Jinming Wang
YL Youquan Li
GG Guiquan Guan
JL Jianxun Luo
HY Hong Yin
GL Guangyuan Liu
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Nucleotide sequences were aligned using MAFFT v7 (https://mafft.cbrc.jp/alignment/software/), and use ModelFinder (ModelFinder is implemented in IQ-TREE version 1.6.1.2, http://www.iqtree.org) to calculate the best model (K2P + G4 model was selected based on Bayesian Information Criterion: BIC). Bayesian inference (BI) and Monte Carlo Markov Chain (MCMC) methods were used to construct the phylogenetic tree in PhyloSuite v1.2.2 (https://github.com/dongzhang0725/PhyloSuite/ releases), The number of substitutions (Nst) was set at two, and posterior probability values were calculated by running 2,000,000 generations with four simultaneous tree-building chains. A 50% majority-rule consensus tree was constructed from the final 75% of the trees generated by BI. Analyses were run three times to ensure convergence and insensitivity to priors. Phylogenetic tree were edited in Figtree v1.4.3 (https://github.com/rambaut/figtree/releases).

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