4.4. Average Molecular Mass Analysis of Extracted Xylan

The high-performance size exclusion chromatography (HP-SEC) analysis of extracted xylan from Acacia sawdust was performed for the determination of average molecular mass. Extracted xylan (10 μL, 1 mg/mL) and dextran standard molecular weight markers, i.e., 10, 20, 40, 70, 100, 200, 270, and 500 kDa, at a concentration of 1 mg/mL were loaded on a Phenomenex Polysep-GFC-P6000 column coupled with a guard column (Phenomenex Polysep-GFC-P) connected to an HPLC system equipped with an RI detector. The loaded samples were eluted at a flow rate of 0.5 mL/min using a 100 mM NaNO₃ solution as a mobile phase. The molecular mass of extracted xylan was calculated by a Hendricks plot using dextran standards.