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Dharmacon SMARTpool ON-TARGETplus siRNAs were added to cells cultured in six-well plates (30 pmol IP6K1 siRNA [L-006737] and 30 pmol IP6K2 siRNA [L-006738]), using 3 µL of the transfection reagent Lipofectamine 3000 (Invitrogen; L3000015) in 100 µL of Opti-MEM reduced serum medium (Gibco; 31985070). Control cells were transfected with Dharmacon ON-TARGETplus GAPD Control Pool (D-001830-10). The transfection was repeated after 24 h, and cells were analyzed at 48 h.
Copyright and License information: ©2020
Published under the PNAS license.
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