Statistical analyses and graphs of the results were performed on GraphPad Prism 7.0 (GraphPad Software, San Diego, CA, USA). Two-tailed t test or one-way ANOVA were used to compare interrelated samples. While two-way ANOVA was used to compare multiple factors. Confidence intervals were fixed at 95% (p < 0.05), 99% (p < 0.01) and 99.9% (p < 0.001). Data are reported as mean ± standard error of the mean (SEM). The number of independent experiments and any other specific information regarding the statistical test used and significance of the differences are specified in the figure legends.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.