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MCF7/Par and T47D/Par cells were lysed using RIP assay buffer (Millipore, Billerica, MA, USA), and then incubated with magnetic beads coated with anti-Ago2. Normal mouse IgG was served as a negative control. Finally, purified RNA was subjected to qRT-PCR analysis to determine the presence of the binding targets.
Copyright and License information: Med Sci Monit, ©2020
This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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