GBM Tissues and Cell Lines

We collected 76 pairs of primary GBM and adjacent normal tissues from The First Affiliated Hospital of Zhengzhou University. All specimens were histologically confirmed by two independent experienced pathologists. The detailed clinical information of patients is summarized in Table 1. Patients were followed up with routinely after surgery, and each patient provided informed consent. This study was approved by the Ethics Committee of The First Affiliated Hospital of Zhengzhou University. A total of six GBM cell lines (U87MG, U251MG, SHG44, Ln229, T98, and A172) and one normal human astrocyte cell line NHA were obtained from Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA) complete medium containing 10% fetal bovine serum (FBS) (Gibco, Invitrogen, Carlsbad, CA, USA), penicillin, and streptomycin.

Correlation Between PCED1B-AS1 Expression and Clinicopathological Features in GBM Patients (n = 76).

GBM: glioblastoma; PCED I B-AS1: PCED1B antisense RNA 1.