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LG Histology and Quantitative Analysis of Lymphocytic Infiltration

SK Shruti Singh Kakan
SJ Srikanth R. Janga
BC Benjamin Cooperman
DC David W. Craig
ME Maria C. Edman
CO Curtis T. Okamoto
SH Sarah F. Hamm-Alvarez
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Lymphocytic infiltration in mouse lacrimal glands, indicative of autoimmune dacryoadenitis, was confirmed and quantified with hematoxylin & eosin staining as described (50). Briefly, lacrimal glands from NOD and BALB/c mice were fixed in 10% NBF (Richard-Allan Scientific, Kalamazoo, MI), fixed in paraffin, then cut into 5 μ horizontal sections and stained with hematoxylin and eosin. Sections were imaged using an Aperio Digital ScanScope (Leica Biosystems Inc., Buffalo Grove, IL) using the 40x objective lens. The percentage of lymphocytic infiltration in the tissue was determined by calculating the area of infiltrates manually using ImageJ (National Institutes of Health, http://imagej.nih.gov/ij). Data were analyzed by GraphPad Prism using one-way non-parametric ANOVA (Kruskal-Wallis).

Copyright and License information:  ©2020 Kakan, Janga, Cooperman, Craig, Edman, Okamoto and Hamm-Alvarez.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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