A fasting blood sample was collected by a standardized procedure. Blood was collected by venipuncture into SST tubes. The serum was separated by centrifugation at 2000 × g for 15 min at 4 °C within 30 min of collection, and samples were immediately stored at -80 °C until analysis. The Diazyme Enzymatic Homocysteine (Hcy) Assay reagent kits were used for the estimation of homocysteine levels by an enzymatic method.
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