4.2. Streptozotocin (STZ)-Induced Diabetes Mellitus in a Rodent Wounding Model

Wistar rodents with diabetes were actuated by a single intraperitoneal injection of STZ (50 mg/kg; Sigma-Aldrich, St. Louis, MO, USA) following our previous report [9,10,25]. Briefly, rodents with a glucose level more prominent than 300 mg/dl one week after injection were characterized as having effective acceptance of diabetes and were then utilized for subsequent experiments. To adjust the glucose level at 200 mg/dL, diabetic rodents were subcutaneously administered continual-acting insulin (1 to 2 unit/kg; Montards Novo Nordisk A/S, Bagsvaerd, Demark) until the animals were immolated. The wounding model was assessed a month after the STZ injection. The dorsum skin tissue of the Wistar rodents was excised to make a wounding area of 6 × 5 cm². The whole skin was disfigured underneath the level of the dorsal fascia, and the edge of the wound defect was stitched set up with 4-0 silk stitches to forestall wound contracture. The wound was incidentally secured with lucent Tegaderm (3M HealthCare, Borken, Germany) till ESWT was initiated.