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Cell Culture and 5-Azacytidine Treatment
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Five HCC cell lines (Huh 7, HCCLM3, HepG2, SMMC-7721, SK-Hep-1) were purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences and was cultured in Minimum Essential Media (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin in an incubator at 37 °C and 5% CO2 concentration. For methylation regulation analysis, cells were treated with 5-Azacytidine (AZA; Selleckchem) at a concentration of 5 μM in the medium for 96 hours.
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This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://www.creativecommon.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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