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4.7. Measurement of Gene Expression by Real-Time PCR

XW Xiong Weng
DL De Lin
JH Jeffrey T. J. Huang
RS Roland H. Stimson
DW David H. Wasserman
LK Li Kang
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Quantitative PCR were performed using the TaqMan® Gene Expression assay (ThermoFisher, Perth, UK) for mouse collagen24α1 (Mm013237744_m1), human collagen24α1 (Hs00537706_m1), mouse collagen1α1 (Mm00801666_g1), mouse collagen1α2 (Mm00483888_m1), mouse collagen3α1 (Mm00802331_m1). 18s (Hs99999901_s1) was used as reference controls in human and mouse adipose tissue. Mouse GAPDH (Mm99999915_g1) was used as reference control in the other tissues.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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