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Time–kill curves of S. aureus ATCC 25923 and MRSA isolate 18032913 were carried out following the procedure described by CLSI [53]. Concentration equal to MIC, twice the MIC and four times the MIC of the extracts were prepared. An inoculum size of 2 × 106 CFU/mL was conducted on Mueller Hinton broth (Sigma, Spain). Aliquots of 0.5 mL of the medium were taken at time intervals of 0, 4, 8 10, 12, and 24h. A graph of the log CFU/mL was plotted against time.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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