Model building and refinement

JD Jia Duan
DS Dan-dan Shen
XZ X. Edward Zhou
PB Peng Bi
QL Qiu-feng Liu
YT Yang-xia Tan
YZ You-wen Zhuang
HZ Hui-bing Zhang
PX Pei-yu Xu
SH Si-Jie Huang
SM Shan-shan Ma
XH Xin-heng He
KM Karsten Melcher
YZ Yan Zhang
HX H. Eric Xu
YJ Yi Jiang
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The cryo-EM structure of PTH1R–Gs–Nb35 complex (PDB code 6NBF) was used as the start for model rebuilding and refinement against the electron microscopy map. The model was docked into the electron microscopy density map using Chimera44, followed by iterative manual adjustment and rebuilding in COOT45. Real space refinement was performed using phenix.real_space_refine from Phenix program package46. The model statistics were validated using MolProbity47. Structural figures were prepared in Chimera and PyMOL (https://pymol.org/2/). The final refinement statistics are provided in Supplementary Table 1.

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