The cryo-EM structure of PTH1R–Gs–Nb35 complex (PDB code 6NBF) was used as the start for model rebuilding and refinement against the electron microscopy map. The model was docked into the electron microscopy density map using Chimera44, followed by iterative manual adjustment and rebuilding in COOT45. Real space refinement was performed using phenix.real_space_refine from Phenix program package46. The model statistics were validated using MolProbity47. Structural figures were prepared in Chimera and PyMOL (https://pymol.org/2/). The final refinement statistics are provided in Supplementary Table 1.
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