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MTT assay

MK Masahiro Kuragano
RY Ryota Yamashita
YC Yusaku Chikai
RK Ryota Kitamura
KT Kiyotaka Tokuraku
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PC12 cells were plated at 2.0 × 104 cells in poly-D-lysine-coated 96-well plates (IWAKI, Japan) and incubated for 24 h. After incubation, cells were treated with 4.5 ng/ml NGF and further incubated for 24 h. Then, cells were treated with QD, QDAβ, and Aβ42 and cells were incubated for a further 24 h. Then, cells were treated with 1.2 mM MTT and incubated for 4 h. After incubation, the supernatant of each well was removed and then crystals of formazan were dissolved in 10% SDS/0.01 M HCl solution. After overnight incubation, absorbance intensity (570 nm) was measured in a 96-well plate. Cell viability was assessed as a percentage relative to DMSO-treated cells.

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