Western blot

The protein was extracted with RIPA lysis buffer containing cocktail and quantified with a BCA kit (23225, Thermo Fisher Scientific). Protein extracts were separated by 10% Next Gel (1B1724, Amresco), then transferred to a PVDF membrane (Immobilon-P, Millipore). The membrane was blocked with 5% skim milk for 2 h at room temperature, incubated with a primary antibody overnight at 4 °C and a secondary antibody for 2 h at room temperature. The membrane was washed four times with TBST for 10 min each time and finally detected with the LAS4000 mini system (GE Healthcare, Piscataway, NJ, USA). The antibodies used are listed in Table S3.