A RIL population consisting of 212 F12 lines derived from the parental cross combination between lines Zheng8903 and cultivar Yuhua4 was used in this study. The female parent Zheng8903 with the pedigree ‘79–266//71–31/Chico’ is a breeding line showing high level of resistance to peanut web blotch [37]. The male parent Yuhua4 is a variety released in 1991 by the Henan Academy of Agricultural Sciences and we obtained the seeds from our own inventory [38], showing high level of susceptibility to web blotch [37]. All the plant materials including RILs and its parents mentioned above were developed and preserved in the corresponding author’s lab.
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