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Guide RNAs targeting MCM8IP, MCM8, and MCM9 were designed using GPP sgRNA Designer (Broad Institute). The targeted sequences are as follows: MCM8IP #1 (5′-CGACCCCCCTTGAGACCTGGT-3′), MCM8IP #2 (5′-TTCAGTATTGGCTAAAAAAGC-3′), MCM8IP #3 (5′-CAGCTGGATTGGCAATCAGAG-3′), MCM8 #1 (5′-CACGTGGCGTGTATGTTTGT-3′), MCM8 #2 (5′-GTGTGTCGAGGCAGGTCATT-3′), MCM9 #1 (5′-ACGGGATTGTAATGCAACGG-3′), and MCM9 #2 (5′-ACACTGTCTGATGTGGGCAA-3′). MCM8IP sgRNAs were cloned into the BsmBI/Esp3I sites of pXPR206 (Addgene #96920). MCM8 and MCM9 sgRNAs were cloned into the BsmBI/Esp3I sites of pLentiCRISPR v2 Blast (Addgene #98293). Following stable lentiviral transduction of cells, targeting efficiencies were evaluated by western blotting.
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