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Lentivirus production and transduction
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Lentiviral vector and packaging plasmids were co-transfected into Lenti-293 cells using Lipofectamine 2000, respectively. The recombinant lentiviruses were harvested 48h after transfection. Breast cancer cells were infected with recombinant lentivirus-transducing units plus 5μg/ml Polybrene (Sigma-Aldrich). Stable clones were obtained using relevant agents for selection.
Copyright and License information: ©2017 Zhan et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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