2.4. Oxygen Radical Absorbance Capacity (ORAC) Assay

According to Moudache et al. [9], 25 μL of different concentrations of (0.01–0.2 mg/mL) CAE were incubated in a 96-well microplate with 125 μL of fluorescein (10 nM in 75 mM NaH₂PO₄ buffer at pH 7.4) for 30 min at 37 °C. Then, 25 μL of 10 mM AAPH was added to each well and fluorescence was recorded (λ_ex 485 nm and λ_em 520 nm) every 2 min for 90 min using a GLOMAX Multidetection System (Promega, Madison, WI, USA). Trolox (0–100 μM) was used as the reference standard. Results were calculated on the basis of differences in areas under the fluorescence decay curve between the blank, samples, and standards. Final oxygen radical absorbance capacity (ORAC) values were expressed as μmol of Trolox equivalents (TE)/100 g of dried extract (DE).