4.16. Macrophage Phagocytosis Assay

HMDMs were isolated, differentiated, and polarized as described above for 24 h. Polarized cells were treated with 1 μM thioA for 30 min. After incubation for 15 min with fluorescent latex beads (50 beads/cell, Fluoresbrite carboxylated YG microspheres, 1.75 μm, Polyscience, Warrington, England, UK, #17687), macrophages were washed four times with cold PBS and detached from plates using PBS containing 5 mM EDTA. Cells were resuspended in FACS wash and examined on a BD LSRFortessa using BD FACSDiva software (BD Biosciences).