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Roughly, the 50% inhibitory concentration (IC50) of Poly(I:C) on NHKs was determined by CCK8 kit (7Sea biotech, China) according to the manufacturer’s instructions. In general, seeded in 96-well culture plates, cells were treated with 0-4 μg/ml Poly(I:C) for 24 h. Next, incubated with 100 μl fresh medium with 10 μl CCK8 solution for 2 h at 37 °C and optical density (OD) was measured at 450 nm by Model 680 Microplate Reader (Bio-Rad, USA).
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