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Primary cell isolation

GS Gabriel Sollberger
RS Robert Streeck
FA Falko Apel
BC Brian Edward Caffrey
AS Arthur I Skoultchi
AZ Arturo Zychlinsky
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Neutrophils were purified by centrifugation of whole blood over Histopaque-1119 (Sigma), followed by centrifugation over a discontinuous Percoll gradient (Zhang et al., 1997). PBMCs were collected from the upper phase after Histopaque-1119 centrifugation, washed and either used for RNA extraction or for isolation of monocytes by positive selection (CD14) with magnetic beads (130-050-201, Miltenyi Biotec). Eosinophils were isolated from full blood using an isolation kit according to the manufacturer’s instructions (130-092-010, Miltenyi Biotec).

Copyright and License information:  ©2020, Sollberger et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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