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To assess the cell size of NMVCs, the cultured NMVCs were fixed in 3.7% formaldehyde and permeabilized in 0.1% Triton X-100 for 10 min, respectively, followed by incubation with blocking solution for 40 min and subsequently with FITC-phalloidin (10 μg/ml, Sigma-Aldrich) at 37°C. Confocal micrographs were obtained using a Leica SP5.
Copyright and License information: ©2019 The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Cardiology
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