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Cardiac energetics

RN Rianne Nederlof
EG Ebru Gürel-Gurevin
OE Otto Eerbeek
CX Chaoqin Xie
GD G. Sjoerd Deijs
MK Moritz Konkel
JH Jun Hu
NW Nina C. Weber
CS Cees A. Schumacher
AB Antonius Baartscheer
EM Egbert G. Mik
MH Markus W. Hollmann
FA Fadi G. Akar
CZ Coert J. Zuurbier
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Cardiac energetics was measured in Langendorff perfused rat hearts (n = 4–6 per group). Hearts were exposed for 20 min to 1 μM TAT-only or 200 nM TAT-HKII. Thereafter, for one group, hearts were snap frozen in liquid nitrogen, weighed and stored at −80 °C until analysis. Another group was exposed to 15-min ischemia followed by 7-min reperfusion, after which the hearts were frozen.

Hearts were freeze-dried overnight and dry weight was determined. ATP, PCr, creatinine (Cr) and inorganic phosphate (Pi) were determined as described by Fiolet et al. [9]. The phosphorylation potential (ΔGATP) was calculated from these values.

Copyright and License information: The Author(s) ©2017
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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