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Prussian blue staining

JS Jesse D. Slone
LY Li Yang
YP Yanyan Peng
LQ Luis F. Queme
BH Belinda Harris
SR Stacey J. Sukoff Rizzo
TG Torrian Green
JR Jennifer L. Ryan
MJ Michael P. Jankowski
LR Laura G. Reinholdt
TH Taosheng Huang
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Mice were euthanized in age-matched groups and perfused with 0.9% saline. Frozen tissue sections fixed with 4% PFA were placed in working Iron Stain Solution (Sigma-Aldrich; St. Louis, MO, HT20-1KT). The staining solution consisted of an equal volume of potassium ferrocyanide solution and hydrochloride acid solution. Tissues were stained for 10 min, rinsed in deionized water, and stained in working pararosaniline solution (1 ml of pararosaniline solution added to 50 ml water) for 5 min. The stained tissues were rinsed in deionized water, rapidly dehydrated through alcohol and xylene, and then mounted.

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