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Cells were trypsinized, washed three times with PBS, and fixed with 70% ethanol for 12 h at 4 °C. After washing twice with PBS, the fixed cells were resuspended in PI/RNase staining buffer (BD Biosciences, San Jose, CA, USA) for 10 min in the dark at 37 °C before analysis. Cell cycle analysis was performed using a FACSCalibur flow cytometer (BD Biosciences).
Copyright and License information: ©2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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