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Measure of Minimal Inhibitory Concentration (MIC)
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The effect of Ca37 mAb in combination with amphotericin B and fluconazole was studied following the guidelines of EUCAST42 using C. albicans CECT 13062 and fluconazole-resistant UPV 93–153 strains, and 20, 10, 2, 1 and 0 µg/mL concentrations of antibody.
The plates were incubated for 24 or 48 h at 37 °C, and absorbance recorded at 530 nm. The FICI was calculated using the derived MICs according to Bardbari et al.43.
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