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KEGG Orthology (KO) annotation

DD D. Derilus
MR M. Z. Rahman
FP F. Pinero
SM S. E. Massey
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The KEGG Orthology (KO) assignment was conducted for each individual genome separately. This was done by a Blastp search of the protein *fasta file for each species against the non-redundant protein NCBI database, with an e-value cut-off of 1e−10. From this blast output, the gene ID and Genbank ID numbers (GI) were retrieved and sorted. The resulting GI numbers were converted to UniProt and then to K numbers subsequently using an in-house ID mapping python script that can be obtained from github.com/dieunelderilus/picoeukaryotes/blob/master/gi_kO_mapper.py. Briefly, this script takes as input a table with gene ID and GI numbers for the considered species and outputs a comma separated table which links each individual gene ID to its corresponding UniProt and K number respectively (GeneID→GI→UniProt ID→K).

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