Gene annotation and sequence analysis

SH Shuilian He
YY Yang Yang
ZL Ziwei Li
XW Xuejiao Wang
YG Yanbing Guo
HW Hongzhi Wu
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The CpGAVAS pipeline (Liu et al., 2012) was used to annotate the genome and start/stop codons and intron/exon boundaries were adjusted in Geneious 8.1 (Kearse et al., 2012). The tRNA was identified using tRNAscan-SE v2.0 (Lowe & Chan, 2016), and sequence data were subsequently deposited in GenBank. The online tool OGDraw v1.2 (http://ogdraw.mpimp-golm.mpg.de/, Lohse, Drechsel & Bock, 2007) was used to generate a physical map of the genome.

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