Mice were required to be fasted prior to assay using a blood glucose meter (Roche ACCU-CHEK, Germany), and fasting blood glucose levels were measured once a week. Mouse tail vein blood was collected for these samples, while whole blood was collected at the conclusion of the experiment, and serum was separated for further analysis. Serum TC and TG were measured using an automatic biochemical analyzer (Shenzhen Leidu Life Technology Co., Ltd.). Measurement of serum FFA was performed using an UV-Vis 752 spectrophotometer (Lengguang Optical Instrument, Shanghai, China).
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