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Macrophages differentiated from THP-1 cells were cultured in the absence/presence of MCF-7/T47D cells by using a 0.4 µm transwell system (Sigma-Aldrich). After co-culture for 5 days, macrophages and breast cancer cells were collected for further study.
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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