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3.2. Assessment of the MCT1 Inhibition by [14C]lactate Uptake Assay
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Inhibition of the MCT1-mediated lactate transport was determined using the rat brain endothelial cell line (RBE4, a gift from F. Roux research group [40]) as previously described [26]. PCR analysis and Western Blot demonstrated that only the MCT1 isoform is expressed by RBE4 cells.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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