Human colorectal carcinoma cell lines (HCT116, Homo Sapiens colon colorectal carcinoma, ATCC, Manassas, VA, USA), were used to study the promoter methylation of SFRP2 under treatment with vitamin D. Cells were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, penicillin, and streptomycin at 37 °C and 5% CO2. Cell lines were treated with the vitamin D (Sigma Aldrich, Madrid, Spain) at 10 nM and 100 nM for 2, 6, 12, 24, 48, and 72 h.
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